Evaluation of Peri-Implantitis through Fourier-Transform Infrared Spectroscopy on Saliva
Background: Peri-implantitis is characterized as a pathological change in the tissues around dental implants. Fourier-transform infrared spectroscopy (FTIR) provides molecular information from optical phenomena observed by the vibration of molecules, being used in biological studies to characterize changes and serving as a form of diagnosis. Aims: this case-control study evaluated the peri-implant disease by using FTIR spectroscopy with attenuated total reflectance (ATR) in the fingerprint region. Methods: forty saliva samples were evaluated, 18 from the control group and 22 from the peri-implantitis group. Clinical data such as plaque index (PI), gingival index (GI), probing depth (PS) and attachment level (AL) were assessed. Results: The results of clinical parameters showed statistical difference between the two groups regarding an excess of the plaque index (PI). In the FTIR-ATR analysis, the main components revealed vibrational modes of fatty acids, histidine, lipid esters, nucleic acids and tryptophan, with the main molecules contributing to spectral discrimination. The five-component PLS-DA classification model had an accuracy of 81%, showing differences between healthy and diseased implants. Conclusion: the FTIR spectroscopy provides important molecular characteristics of the samples and that the results in association with clinical data show the effectiveness of using this tool for diagnosing the disease.
Citação
@online{graziele2024,
author = {Graziele , Parize and Gabriele , Nepomuceno and Jamil ,
Shibli and Caetano, Matheus, Willian and Kim, Yeon, Jung and Paulo
Henrique , Braz-Silva and Herculano , Martinho and Debora , Pallos},
title = {Evaluation of Peri-Implantitis through Fourier-Transform
Infrared Spectroscopy on Saliva},
date = {2024-12-21},
doi = {10.5281/ZENODO.14540221},
langid = {pt-BR},
abstract = {Background: Peri-implantitis is characterized as a
pathological change in the tissues around dental implants.
Fourier-transform infrared spectroscopy (FTIR) provides molecular
information from optical phenomena observed by the vibration of
molecules, being used in biological studies to characterize changes
and serving as a form of diagnosis. Aims: this case-control study
evaluated the peri-implant disease by using FTIR spectroscopy with
attenuated total reflectance (ATR) in the fingerprint region.
Methods: forty saliva samples were evaluated, 18 from the control
group and 22 from the peri-implantitis group. Clinical data such as
plaque index (PI), gingival index (GI), probing depth (PS) and
attachment level (AL) were assessed. Results: The results of
clinical parameters showed statistical difference between the two
groups regarding an excess of the plaque index (PI). In the FTIR-ATR
analysis, the main components revealed vibrational modes of fatty
acids, histidine, lipid esters, nucleic acids and tryptophan, with
the main molecules contributing to spectral discrimination. The
five-component PLS-DA classification model had an accuracy of 81\%,
showing differences between healthy and diseased implants.
Conclusion: the FTIR spectroscopy provides important molecular
characteristics of the samples and that the results in association
with clinical data show the effectiveness of using this tool for
diagnosing the disease.}
}